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Issue 17, 2016
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Reprogrammable multiplexed detection of circulating oncomiRs using hybridization chain reaction

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Abstract

In this study, we have coupled the DNA polymerization capability of hybridization chain reaction (HCR) with the plasmonic properties of gold nanoparticles to develop a reprogrammable and multiplexed detection of three circulating oncomiRs (miR-10b, miR-21 and miR-141) dysregulated in various disease states of breast cancer. We have demonstrated that by simply changing the initiator (label-free short single stranded DNA) content of the HCR, while keeping everything else unchanged, the same nanoparticle assembly can be reprogrammed for the detection of the target oncomiRs individually or simultaneously in all possible combinations. We have shown that as little as 20 femtomoles of each oncomiR can be detected visually without using any analytical instrument. Furthermore, we demonstrated that the target oncomiR can be detected in an RNA pool isolated from a liquid biopsy mimic of breast cancer.

Graphical abstract: Reprogrammable multiplexed detection of circulating oncomiRs using hybridization chain reaction

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Publication details

The article was received on 01 Dec 2015, accepted on 25 Jan 2016 and first published on 25 Jan 2016


Article type: Communication
DOI: 10.1039/C5CC09910B
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2016,52, 3524-3527
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    Reprogrammable multiplexed detection of circulating oncomiRs using hybridization chain reaction

    M. Rana, M. Balcioglu, M. Kovach, M. S. Hizir, N. M. Robertson, I. Khan and M. V. Yigit, Chem. Commun., 2016, 52, 3524
    DOI: 10.1039/C5CC09910B

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