Issue 12, 2016
From the journal:

Chemical Communications

One-pot peptide and protein conjugation: a combination of enzymatic transamidation and click chemistry†

N. M. Rachelabc  and  J. N. Pelletier*abcd  

* Corresponding authors

a Department of Chemistry, Université de Montréal, 2900 Boulevard Edouard-Montpetit, Montréal, Québec, Canada
E-mail: joelle.pelletier@umontreal.ca

b PROTEO, the Québec Network for Function, Structure and Engineering, Québec, Canada

c CGCC, the Center for Green Chemistry and Catalysis, Montréal, Québec, Canada

d Department of Biochemistry, Université de Montréal, 2900 Boulevard Edouard-Montpetit, Montréal, Québec, Canada

Abstract

Enzymatic transamidation and copper-catalyzed azide–alkyne cycloaddition (CuAAC) were combined to yield covalently conjugated peptides and proteins. The addition of glutathione preserved enzymatic activity in the presence of copper. Tuning the reaction kinetics was key to success, providing up to 95% conversion. This one-pot reaction allowed for targeted fluorescent protein labeling.

Graphical abstract: One-pot peptide and protein conjugation: a combination of enzymatic transamidation and click chemistry

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC09163B
Article type
Communication
Submitted
04 Nov 2015
Accepted
15 Dec 2015
First published
04 Jan 2016

Chem. Commun., 2016,52, 2541-2544

Permissions

Request permissions

One-pot peptide and protein conjugation: a combination of enzymatic transamidation and click chemistry

N. M. Rachel and J. N. Pelletier, Chem. Commun., 2016, 52, 2541 DOI: 10.1039/C5CC09163B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements