All-in-One azides: empowered click reaction for in vivo labeling and imaging of biomolecules†
Abstract
We designed and synthesized All-in-One (AIO) reactive azide reagents for bioorthogonal reactions with highly efficient Cu(I) ligand moieties, an azido group, and functional tags for imaging or purification. The AIO reagents displayed fast and efficient click ligation and can be applied in a wide range of in vivo systems.