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Issue 43, 2016
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One-pot construction of Quenchbodies using antibody-binding proteins

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Abstract

Fluorolabeled antibody-binding proteins were constructed based on Staphylococcus protein A and Streptococcus protein G domains, and used as an adaptor to convert the Fab fragment of interest to a Q-body, a fluorescent biosensor that exhibits antigen-dependent fluorescence enhancement. Without having to perform the tedious procedure of genetically introducing a fluorescent dye molecule into a cloned Fab fragment, we successfully converted both a cloned anti-osteocalcin Fab fragment and a commercially available anti-vimentin (Fab)2 fragment to a Q-body using this method. This method is not only a simpler way for constructing Q-bodies but also a convenient alternative to finding a suitable antibody that has a greater potential to become an excellent biosensor.

Graphical abstract: One-pot construction of Quenchbodies using antibody-binding proteins

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Publication details

The article was received on 25 Jul 2016, accepted on 02 Oct 2016 and first published on 05 Oct 2016


Article type: Paper
DOI: 10.1039/C6AY02108E
Citation: Anal. Methods, 2016,8, 7774-7779
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    One-pot construction of Quenchbodies using antibody-binding proteins

    H. Jeong, T. Kojima, J. Dong, H. Ohashi and H. Ueda, Anal. Methods, 2016, 8, 7774
    DOI: 10.1039/C6AY02108E

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