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Issue 42, 2016
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Characterization of TLR4/MD-2-modified Au sensor surfaces towards the detection of molecular signatures of bacteria

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Abstract

Lipopolysaccharides (LPSs), also known as endotoxins, can be fatal even at low concentrations. As a result, the development of novel methodologies for LPS detection has been continuously in the focus of research. Biosensors, which employ a bio-recognition element on a transducer surface, are on the cutting edge of these novel technologies. In this report, Au surfaces modified with TLR4/MD-2 through Lip-NHS linkers with an ultimate potential application as biosensors for LPS detection have been characterized and investigated using X-ray photoelectron spectroscopy, quartz crystal microbalance and electrochemical techniques. Also the interaction between TLR4/MD-2 immobilized on Au surfaces with LPSs has been studied to evaluate the possibility of LPS detection.

Graphical abstract: Characterization of TLR4/MD-2-modified Au sensor surfaces towards the detection of molecular signatures of bacteria

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Publication details

The article was received on 12 Jul 2016, accepted on 21 Sep 2016 and first published on 22 Sep 2016


Article type: Paper
DOI: 10.1039/C6AY01978A
Citation: Anal. Methods, 2016,8, 7623-7631
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    Characterization of TLR4/MD-2-modified Au sensor surfaces towards the detection of molecular signatures of bacteria

    K. Amini, I. I. Ebralidze, N. W. C. Chan and H. Kraatz, Anal. Methods, 2016, 8, 7623
    DOI: 10.1039/C6AY01978A

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