Jump to main content
Jump to site search

Issue 43, 2016
Previous Article Next Article

An automatic and sensitive method for simultaneous determination of sixteen polycyclic aromatic hydrocarbons and four derivatives by online solid-phase extraction coupled with ultra-high performance liquid chromatography with online solvent filtration

Author affiliations

Abstract

An automatic and sensitive method for simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 4 derivatives by online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography (UHPLC) with online solvent filtration in groundwater samples was developed. Groundwater samples were injected directly after filtration and automatic preconcentration into a C18 online SPE column. After the valve switching, the analytes were eluted in the forward-flush mode and then separated on a UHPLC column. Chromatographic parameters, cleanup conditions, elution time, sample loading flow rate and selection of online SPE columns were compared and optimized in detail. An online precolumn for solvent filtration was effective in reducing background interference. The separation resolution and peak shape were sharply improved by adding 1ā€° formic acid in the elution mobile phase. Complete separation was obtained for all the analytes. An aliquot of 2.4 mL groundwater samples was sufficient for sensitive analysis with fluorescent detectors (FLDs). The method recoveries ranged from 60.0% to 126.0% and the interday and intraday relative standard deviations (RSDs) ranged from 1.1% to 7.5%. The limits of detection (LODs) were between 0.1 and 35.0 ng Lāˆ’1. The total analysis time including the pretreatment procedure was only 21.0 min. The developed method was successfully applied to the analysis of groundwater samples for routine surveys. The proposed method provides an alternative way to automatic and simultaneous determination of 16 PAHs and 4 derivatives in groundwater, especially for routine monitoring of batch groundwater samples.

Graphical abstract: An automatic and sensitive method for simultaneous determination of sixteen polycyclic aromatic hydrocarbons and four derivatives by online solid-phase extraction coupled with ultra-high performance liquid chromatography with online solvent filtration

Back to tab navigation

Publication details

The article was received on 29 Jun 2016, accepted on 30 Aug 2016 and first published on 31 Aug 2016


Article type: Paper
DOI: 10.1039/C6AY01846G
Citation: Anal. Methods, 2016,8, 7721-7730
  •   Request permissions

    An automatic and sensitive method for simultaneous determination of sixteen polycyclic aromatic hydrocarbons and four derivatives by online solid-phase extraction coupled with ultra-high performance liquid chromatography with online solvent filtration

    F. Guo, Z. Rao, N. Zhan, G. Li, X. Guo, C. Liu, Z. Yang, S. Song and X. Zuo, Anal. Methods, 2016, 8, 7721
    DOI: 10.1039/C6AY01846G

Search articles by author

Spotlight

Advertisements