Jump to main content
Jump to site search

Issue 1, 2017
Previous Article Next Article

Real-time femtomolar detection of cancer biomarkers from photoconjugated antibody–phage constructs

Author affiliations

Abstract

Here we describe novel covalent conjugates of antibody–phage for the detection of multiple cancer biomarkers using real time immuno-polymerase chain reaction (immuno-PCR). While the conventional process of immuno-PCR utilizes DNA-conjugated antibodies, chemical modification of antibodies not only reduces antibody affinity but also creates a heterogeneous population of products. However, phage naturally encapsulate genomic DNA, which can be used as a PCR template. To produce covalently conjugated antibody–phage constructs without recombinant antibody expression or chemical modification of antibodies, we incorporated a photocrosslinkable non-canonical amino acid within an antibody-binding domain displayed on one of the phage coat proteins. To correlate antigen presence to a specific DNA sequence, the phage genomes were modified with domains that recognized specific sets of primers. The crosslinked antibody–phage conjugates were then tested in a sandwich-type immunoassay using real-time PCR where low pg ml−1 concentrations of antigen could be detected and identified from a single solution containing a mixture of three different types of cancer biomarkers.

Graphical abstract: Real-time femtomolar detection of cancer biomarkers from photoconjugated antibody–phage constructs

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 25 Aug 2016, accepted on 02 Oct 2016, published on 04 Oct 2016 and first published online on 04 Oct 2016


Article type: Paper
DOI: 10.1039/C6AN01904H
Citation: Analyst, 2017,142, 91-97
  •   Request permissions

    Real-time femtomolar detection of cancer biomarkers from photoconjugated antibody–phage constructs

    M. Brasino and J. N. Cha, Analyst, 2017, 142, 91
    DOI: 10.1039/C6AN01904H

Search articles by author