Jump to main content
Jump to site search

Issue 23, 2016
Previous Article Next Article

Lectin-based lateral flow assay: proof-of-concept

Author affiliations

Abstract

Lateral flow assays (LFAs) enable the simple and rapid detection and quantification of analytes and is popular for point-of-care (PoC), point-of-use and outdoor testing applications. LFAs typically depend on antibody or nucleic acid based recognition. We present the innovative concept of a LFA using lectins in the role of the biorecognition element. Lectins are a special kind of glycan-binding protein and the lectin-based LFA herein described was developed for the determination of the glycosylation of free prostate specific antigen (PSA). PSA is routinely used as a biomarker of prostate cancer (PCa) and the glycosylation status of PSA is a more specific marker of disease progress than only the PSA level. Using the lectin-based LFA we were able to detect α-2,6 sialic acid present in fPSA using Sambucus nigra (SNA) lectin. As a negative control, we employed Maackia amurensis lectin II (MAA II) which specifically binds α-2,3 sialic acid. The novel approach presented here can be applied to a wide range of biomarkers that have a significant impact on clinical diagnosis and prognosis, providing an alternative to standard lectin-based assays. The assay uses commercial components and is easily performed by applying a sample to the sampling pad on the lectin-based LFA strip, with results obtained within 10 minutes.

Graphical abstract: Lectin-based lateral flow assay: proof-of-concept

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 02 Aug 2016, accepted on 07 Oct 2016 and first published on 07 Oct 2016


Article type: Paper
DOI: 10.1039/C6AN01746K
Citation: Analyst, 2016,141, 6444-6448
  •   Request permissions

    Lectin-based lateral flow assay: proof-of-concept

    P. Damborský, K. M. Koczula, A. Gallotta and J. Katrlík, Analyst, 2016, 141, 6444
    DOI: 10.1039/C6AN01746K

Search articles by author