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Issue 23, 2016
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Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach

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Abstract

A loss of ability of cells to undergo apoptosis (programmed cell death, whereby the cell ceases to function and destroys itself) is commonly associated with cancer, and many anti-cancer interventions aim to restart the process. Consequently, the accurate quantification of apoptosis is essential in understanding the function and performance of new anti-cancer drugs. Dielectrophoresis has previously been demonstrated to detect apoptosis more rapidly than other methods, and is low-cost, label-free and rapid, but has previously been unable to accurately quantify cells through the apoptotic process because cells in late apoptosis disintegrate, making cell tracking impossible. In this paper we use a novel method based on light absorbance and multi-population tracking to quantify the progress of apoptosis, benchmarking against conventional assays including MTT, trypan blue and Annexin-V. Analyses are performed on suspension and adherent cells, and using two apoptosis-inducing agents. IC50 measurements compared favourably to MTT and were superior to trypan blue, whilst also detecting apoptotic progression faster than Annexin-V.

Graphical abstract: Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach

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Publication details

The article was received on 14 Jul 2016, accepted on 16 Oct 2016 and first published on 19 Oct 2016


Article type: Paper
DOI: 10.1039/C6AN01596D
Citation: Analyst, 2016,141, 6408-6415
  • Open access: Creative Commons BY-NC license
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    Accurate quantification of apoptosis progression and toxicity using a dielectrophoretic approach

    E. A. Henslee, R. M. Torcal Serrano, F. H. Labeed, R. I. Jabr, C. H. Fry, M. P. Hughes and K. F. Hoettges, Analyst, 2016, 141, 6408
    DOI: 10.1039/C6AN01596D

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