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Issue 18, 2016
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Molecular labels for analysis of amines and diols by spray based ionization-mass spectrometry

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Abstract

A family of imidazolium and pyridinium salts was synthesized for the purpose of labeling amines and 1,2-diols for highly sensitivite analysis by mass spectrometry. The chosen mass labels are shown to serve as effective reporters when bound to particles functionalized with amines or 1,2-diols and the binding is reversible. The straightforward synthetic route allows analogous internal standards to be generated quickly. Solvents amenable to electrospray ionization facilitate the rapid liberation of imidazolium and pyridinium mass labels from particles by hydrolysis in aqueous acid, while the acetal bond remains stable in anhydrous or buffered aqueous solution. Detection of the labels is demonstrated at 1 nM and quantitation of mass labels released from particles is also demonstrated.

Graphical abstract: Molecular labels for analysis of amines and diols by spray based ionization-mass spectrometry

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Publication details

The article was received on 18 Apr 2016, accepted on 22 Jun 2016 and first published on 22 Jun 2016


Article type: Paper
DOI: 10.1039/C6AN00907G
Citation: Analyst, 2016,141, 5398-5403
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    Molecular labels for analysis of amines and diols by spray based ionization-mass spectrometry

    S. T. Ayrton, R. G. Cooks and M. Pugia, Analyst, 2016, 141, 5398
    DOI: 10.1039/C6AN00907G

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