Issue 15, 2016

Electrocatalytic monitoring of peptidic proton-wires

Abstract

The transfer of protons or proton donor/acceptor abilities is an important phenomenon in many biomolecular systems. One example is the recently proposed peptidic proton-wires (H-wires), but the ability of these His-containing peptides to transfer protons has only been studied at the theoretical level so far. Here, for the first time the proton transfer ability of peptidic H-wires is examined experimentally in an adsorbed state using an approach based on a label-free electrocatalytic reaction. The experimental findings are complemented by theoretical calculations at the ab initio level in a vacuum and in an implicit solvent. Experimental and theoretical results indicated Ala3(His–Ala2)6 to be a high proton-affinity peptidic H-wire model. The methodology presented here could be used for the further investigation of the proton-exchange chemistry of other biologically or technologically important macromolecules.

Graphical abstract: Electrocatalytic monitoring of peptidic proton-wires

Supplementary files

Article information

Article type
Communication
Submitted
14 Apr 2016
Accepted
14 Jun 2016
First published
15 Jun 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 4554-4557

Electrocatalytic monitoring of peptidic proton-wires

V. Dorčák, M. Kabeláč, O. Kroutil, K. Bednářová and J. Vacek, Analyst, 2016, 141, 4554 DOI: 10.1039/C6AN00869K

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