Issue 13, 2016
From the journal:

Analyst

A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes†

Hui Wen,‡ab   Qinghua Cui,‡c   Hui Meng,c   Fangfang Lai,a   Shufang Wang,b   Xiang Zhang,b   Xiaoguang Chen,a   Huaqing Cui*ab  and  Dali Yin*a  

* Corresponding authors

a State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
E-mail: hcui@imm.ac.cn, yindali@imm.ac.cn

b Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China

c College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, China

Abstract

Cell multinucleation is closely related to chromosomal instability. We report a simple, convenient method to assess cell multinucleation with cytoplasm-localized fluorescent probes (CLFP) which is superior to conventional nuclear staining methods. The CLFP method provides high-resolution images that enable the accurate calculation of the number of nuclear fragments.

Graphical abstract: A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes

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Article information

DOI
https://doi.org/10.1039/C6AN00613B
Article type
Communication
Submitted
15 Mar 2016
Accepted
19 May 2016
First published
19 May 2016

Analyst, 2016,141, 4010-4013

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A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes

H. Wen, Q. Cui, H. Meng, F. Lai, S. Wang, X. Zhang, X. Chen, H. Cui and D. Yin, Analyst, 2016, 141, 4010 DOI: 10.1039/C6AN00613B

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