Issue 12, 2016

Emerging techniques for ultrasensitive protein analysis

Abstract

Many important biomarkers for devastating diseases and biochemical processes are proteins present at ultralow levels. Traditional techniques, such as enzyme-linked immunosorbent assays (ELISA), mass spectrometry, and protein microarrays, are often not sensitive enough to detect proteins with concentrations below the picomolar level, thus requiring the development of analytical techniques with ultrahigh sensitivities. In this review, we highlight the recent advances in developing novel techniques, sensors, and assays for ultrasensitive protein analysis. Particular attention will be focused on three classes of signal generation and/or amplification mechanisms, including the uses of nanomaterials, nucleic acids, and digital platforms.

Graphical abstract: Emerging techniques for ultrasensitive protein analysis

Article information

Article type
Minireview
Submitted
09 Jan 2016
Accepted
08 Feb 2016
First published
08 Feb 2016

Analyst, 2016,141, 3473-3481

Emerging techniques for ultrasensitive protein analysis

X. Yang, Y. Tang, R. R. Alt, X. Xie and F. Li, Analyst, 2016, 141, 3473 DOI: 10.1039/C6AN00059B

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