Jump to main content
Jump to site search

Issue 5, 2016
Previous Article Next Article

A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Author affiliations

Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

Graphical abstract: A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Jan 2016, accepted on 01 Feb 2016 and first published on 05 Feb 2016


Article type: Edge Article
DOI: 10.1039/C6SC00045B
Author version
available:
Download author version (PDF)
Citation: Chem. Sci., 2016,7, 3298-3303
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

    J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298
    DOI: 10.1039/C6SC00045B

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements