Jump to main content
Jump to site search

Issue 17, 2016
Previous Article Next Article

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Author affiliations

Abstract

Multifunctional composite nanoprobes consisting of iron oxide nanoparticles linked to silver and gold nanoparticles, Ag–Magnetite and Au–Magnetite, respectively, were introduced by endocytic uptake into cultured fibroblast cells. The cells containing the non-toxic nanoprobes were shown to be displaceable in an external magnetic field and can be manipulated in microfluidic channels. The distribution of the composite nanostructures that are contained in the endosomal system is discussed on the basis of surface-enhanced Raman scattering (SERS) mapping, quantitative laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping, and cryo soft X-ray tomography (cryo soft-XRT). Cryo soft-XRT of intact, vitrified cells reveals that the composite nanoprobes form intra-endosomal aggregates. The nanoprobes provide SERS signals from the biomolecular composition of their surface in the endosomal environment. The SERS data indicate the high stability of the nanoprobes and of their plasmonic properties in the harsh environment of endosomes and lysosomes. The spectra point at the molecular composition at the surface of the Ag–Magnetite and Au–Magnetite nanostructures that is very similar to that of other composite structures, but different from the composition of pure silver and gold SERS nanoprobes used for intracellular investigations. As shown by the LA-ICP-MS data, the uptake efficiency of the magnetite composites is approximately two to three times higher than that of the pure gold and silver nanoparticles.

Graphical abstract: Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Apr 2016, accepted on 13 Jun 2016 and first published on 13 Jun 2016


Article type: Paper
DOI: 10.1039/C6AN00890A
Citation: Analyst, 2016,141, 5096-5106
  • Open access: Creative Commons BY license
  •   Request permissions

    Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

    T. Büchner, D. Drescher, V. Merk, H. Traub, P. Guttmann, S. Werner, N. Jakubowski, G. Schneider and J. Kneipp, Analyst, 2016, 141, 5096
    DOI: 10.1039/C6AN00890A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements