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Issue 40, 2015
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Collagen-mimetic hydrogels promote human endothelial cell adhesion, migration and phenotypic maturation

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Abstract

This work evaluates the response of human aortic endothelial cells (HAECs) to thromboresistant collagen-mimetic hydrogel coatings toward improving the biocompatibility of existing “off-the-shelf” small-caliber vascular grafts. Specifically, bioactive hydrogels – previously shown to support α12 integrin-mediated cell adhesion but to resist platelet activation – were fabricated by combining poly(ethylene glycol) (PEG) with a 120 kDa, triple-helical collagen-mimetic protein (Scl2-2) containing the GFPGER adhesion sequence. Analysis of HAECs seeded onto the resulting PEG-Scl2-2 hydrogels demonstrated that HAEC adhesion increased with increasing Scl2-2 concentration, while HAEC migration rate decreased over this same concentration range. In addition, evaluation of HAEC phenotype at confluence indicated significant differences in the gene expression of NOS3, thrombomodulin, and E-selectin on the PEG-Scl2-2 hydrogels relative to PEG-collagen controls. At the protein level, however, only NOS3 was significantly different between the PEG-Scl2-2 and PEG-collagen surfaces. Furthermore, PECAM-1 and VE-cadherin expression on PEG-Scl2-2 hydrogels versus PEG-collagen controls could not be distinguished at either the gene or protein level. Cumulatively, these data indicate the PEG-Scl2-2 hydrogels warrant further investigation as “off-the-shelf” graft coatings. In future studies, the Scl2-2 protein can potentially be modified to include additional extracellular matrix or cytokine binding sites to further improve endothelial cell responses.

Graphical abstract: Collagen-mimetic hydrogels promote human endothelial cell adhesion, migration and phenotypic maturation

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Publication details

The article was received on 22 May 2015, accepted on 10 Jul 2015 and first published on 15 Jul 2015


Article type: Paper
DOI: 10.1039/C5TB00990A
Citation: J. Mater. Chem. B, 2015,3, 7912-7919
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    Collagen-mimetic hydrogels promote human endothelial cell adhesion, migration and phenotypic maturation

    D. J. Munoz-Pinto, V. R. Guiza-Arguello, S. M. Becerra-Bayona, J. Erndt-Marino, S. Samavedi, S. Malmut, B. Russell, M. Höök and M. S. Hahn, J. Mater. Chem. B, 2015, 3, 7912
    DOI: 10.1039/C5TB00990A

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