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Issue 25, 2015
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Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

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Abstract

To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 103–105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.

Graphical abstract: Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

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Publication details

The article was received on 26 Feb 2015, accepted on 26 Apr 2015 and first published on 27 Apr 2015


Article type: Communication
DOI: 10.1039/C5TB00381D
Citation: J. Mater. Chem. B, 2015,3, 4997-5000
  • Open access: Creative Commons BY-NC license
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    Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

    S. Löffler and A. Richter-Dahlfors, J. Mater. Chem. B, 2015, 3, 4997
    DOI: 10.1039/C5TB00381D

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