Jump to main content
Jump to site search

Issue 25, 2015
Previous Article Next Article

Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

Author affiliations

Abstract

To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 103–105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.

Graphical abstract: Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Feb 2015, accepted on 26 Apr 2015 and first published on 27 Apr 2015


Article type: Communication
DOI: 10.1039/C5TB00381D
Citation: J. Mater. Chem. B, 2015,3, 4997-5000
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity

    S. Löffler and A. Richter-Dahlfors, J. Mater. Chem. B, 2015, 3, 4997
    DOI: 10.1039/C5TB00381D

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements