Issue 11, 2015

In vivo visualization of osteoarthritic hypertrophic lesions

Abstract

Osteoarthritis (OA) is one of the most common diseases in the aging population. While disease progress in humans is monitored indirectly by X-ray or MRI, small animal OA lesions detection always requires surgical intervention and histology. Here we introduce bimodal MR/NIR probes based on cartilage-targeting 1,4,7,10-tetraazacyclododecane 1,4,7,10-tetraacetic acid amide (DOTAM) that are directly administered to the joint cavity. We demonstrate applications in healthy and diseased rat joints by MRI in vivo. The same joints are inspected post-mortem by fluorescence microscopy, showing not only the precise location of the reagents but also revealing details such as focal cartilage damage and chondrophyte or osteophyte formation. This allows for determining the distinct pathological state of the disease and the regeneration capability of the animal model and will help to correctly assess the effect of potential disease modifying OA drugs (DMOADs) in the future.

Graphical abstract: In vivo visualization of osteoarthritic hypertrophic lesions

Supplementary files

Article information

Article type
Edge Article
Submitted
10 Apr 2015
Accepted
12 Aug 2015
First published
12 Aug 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2015,6, 6256-6261

Author version available

In vivo visualization of osteoarthritic hypertrophic lesions

H. Hu, N. Lim, H. Juretschke, D. Ding-Pfennigdorff, P. Florian, M. Kohlmann, A. Kandira, J. Peter von Kries, J. Saas, K. A. Rudolphi, K. U. Wendt, H. Nagase, O. Plettenburg, M. Nazare and C. Schultz, Chem. Sci., 2015, 6, 6256 DOI: 10.1039/C5SC01301A

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