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Issue 6, 2015
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Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes

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Abstract

Flavin-dependent halogenases are potentially valuable biocatalysts for the regioselective halogenation of aromatic compounds. These enzymes, utilising benign inorganic halides, offer potential advantages over traditional non-enzymatic halogenation chemistry that often lacks regiocontrol and requires deleterious reagents. Here we extend the biocatalytic repertoire of the tryptophan halogenases, demonstrating how these enzymes can halogenate a range of alternative aryl substrates. Using structure guided mutagenesis we also show that it is possible to alter the regioselectivity as well as increase the activity of the halogenases with non-native substrates including anthranilic acid; an important intermediate in the synthesis and biosynthesis of pharmaceuticals and other valuable products.

Graphical abstract: Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes

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Publication details

The article was received on 12 Mar 2015, accepted on 09 Apr 2015 and first published on 10 Apr 2015


Article type: Edge Article
DOI: 10.1039/C5SC00913H
Citation: Chem. Sci., 2015,6, 3454-3460
  • Open access: Creative Commons BY license
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    Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes

    S. A. Shepherd, C. Karthikeyan, J. Latham, A. Struck, M. L. Thompson, B. R. K. Menon, M. Q. Styles, C. Levy, D. Leys and J. Micklefield, Chem. Sci., 2015, 6, 3454
    DOI: 10.1039/C5SC00913H

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