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Issue 7, 2015
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Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

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Abstract

Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by probe JG45.

Graphical abstract: Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

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Publication details

The article was received on 26 Feb 2015, accepted on 30 Apr 2015 and first published on 30 Apr 2015


Article type: Edge Article
DOI: 10.1039/C5SC00718F
Citation: Chem. Sci., 2015,6, 3757-3764
  • Open access: Creative Commons BY license
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    Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

    B. Díaz de Greñu, J. García-Calvo, J. Cuevas, G. García-Herbosa, B. García, N. Busto, S. Ibeas, T. Torroba, B. Torroba, A. Herrera and S. Pons, Chem. Sci., 2015, 6, 3757
    DOI: 10.1039/C5SC00718F

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