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Issue 86, 2015
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Time-resolved confocal microscopy using lanthanide centred near-IR emission

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Abstract

A method to uniquely identify signals originating from probes with different emission decay times in luminescence imaging has been developed. By using scanning confocal microscopy in combination with time-correlated single photon counting (TCSPC), Photon Arrival Time Imaging (PArTI) has been realised through off-line plotting of images using the photon arrival times. PArTI is the time-equivalent to spectrally resolved imaging, replacing the energy axis with a photon arrival time axis. Here, lanthanide probes were used to demonstrate the key advantages of the method. PArTI uses TCSPC data, involves no fitting, uses a single pulsed laser line for multicolour imaging, and can be used with a 100 millisecond dwell time per pixel.

Graphical abstract: Time-resolved confocal microscopy using lanthanide centred near-IR emission

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Publication details

The article was received on 09 Jun 2015, accepted on 10 Aug 2015 and first published on 17 Aug 2015


Article type: Communication
DOI: 10.1039/C5RA15759E
Citation: RSC Adv., 2015,5, 70282-70286
  • Open access: Creative Commons BY-NC license
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    Time-resolved confocal microscopy using lanthanide centred near-IR emission

    Z. Liao, M. Tropiano, S. Faulkner, T. Vosch and T. J. Sørensen, RSC Adv., 2015, 5, 70282
    DOI: 10.1039/C5RA15759E

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