Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 86, 2015
Previous Article Next Article

Time-resolved confocal microscopy using lanthanide centred near-IR emission

Author affiliations

Abstract

A method to uniquely identify signals originating from probes with different emission decay times in luminescence imaging has been developed. By using scanning confocal microscopy in combination with time-correlated single photon counting (TCSPC), Photon Arrival Time Imaging (PArTI) has been realised through off-line plotting of images using the photon arrival times. PArTI is the time-equivalent to spectrally resolved imaging, replacing the energy axis with a photon arrival time axis. Here, lanthanide probes were used to demonstrate the key advantages of the method. PArTI uses TCSPC data, involves no fitting, uses a single pulsed laser line for multicolour imaging, and can be used with a 100 millisecond dwell time per pixel.

Graphical abstract: Time-resolved confocal microscopy using lanthanide centred near-IR emission

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 09 Jun 2015, accepted on 10 Aug 2015 and first published on 17 Aug 2015


Article type: Communication
DOI: 10.1039/C5RA15759E
Citation: RSC Adv., 2015,5, 70282-70286
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Time-resolved confocal microscopy using lanthanide centred near-IR emission

    Z. Liao, M. Tropiano, S. Faulkner, T. Vosch and T. J. Sørensen, RSC Adv., 2015, 5, 70282
    DOI: 10.1039/C5RA15759E

Search articles by author