Issue 72, 2015
From the journal:

RSC Advances

Stability study of tubular DNA origami in the presence of protein crystallisation buffer†

Dianming Wang,‡a   Ziran Da,‡b   Bohan Zhang,a   Mark Antonin Isbell,ab   Yuanchen Dong,a   Xu Zhou,a   Huajie Liu,c   Jerry Yong Yew Heng*b  and  Zhongqiang Yang*a  

* Corresponding authors

a Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
E-mail: zyang@tsinghua.edu.cn

b Surfaces and Particle Engineering Laboratory (SPEL), Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
E-mail: jerry.heng@imperial.ac.uk

c Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

Abstract

This work offers a methodology for screening compatible buffer conditions for both DNA origami and protein crystallisation and studied how protein crystallisation buffer conditions notably cations, buffering agents, precipitants, and pH, influenced the stability of tubular DNA origami.

Graphical abstract: Stability study of tubular DNA origami in the presence of protein crystallisation buffer

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Article information

DOI
https://doi.org/10.1039/C5RA12159K
Article type
Communication
Submitted
02 Jun 2015
Accepted
26 Jun 2015
First published
29 Jun 2015

RSC Adv., 2015,5, 58734-58737

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Stability study of tubular DNA origami in the presence of protein crystallisation buffer

D. Wang, Z. Da, B. Zhang, M. A. Isbell, Y. Dong, X. Zhou, H. Liu, J. Y. Y. Heng and Z. Yang, RSC Adv., 2015, 5, 58734 DOI: 10.1039/C5RA12159K

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