DMSO affects Aβ1–40's conformation and interactions with aggregation inhibitors as revealed by NMR

D. V. Laurents; D. Pantoja-Uceda; L. C. López; J. A. Carrodeguas; M. Mompeán; M. Á. Jiménez; J. Sancho

doi:10.1039/C5RA12100K

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DMSO affects Aβ_1–40's conformation and interactions with aggregation inhibitors as revealed by NMR†

D. V. Laurents,*^a D. Pantoja-Uceda,^a L. C. López,^bc J. A. Carrodeguas,^bc M. Mompeán,^a M. Á. Jiménez^a and J. Sancho*^bc

Author affiliations

* Corresponding authors

^a Instituto de Química Física “Rocasolano”, CSIC, Serrano 119, Madrid, Spain
E-mail: dlaurents@iqfr.csic.es

^b Joint Unit BIFI-IQFR, CSIC, BiocomputationUniversidad de Zaragoza, Mariano Esquillar, Edificio I + D, Zaragoza, Spain

^c Complex Systems Physics Institute (BIFI), Universidad de Zaragoza, Mariano Esquillar, Edificio I + D, Zaragoza, Spain
E-mail: jsancho@unizar.es

Abstract

We show via 3D-heteronuclear NMR spectroscopy that Aβ_1–40 adopts a disordered conformational ensemble with fluctuating turns in DMSO_d6. Using NMR, we map the binding sites of three water-insoluble aggregation inhibitors to Aβ_1–40 in DMSO_d6 and discover remarkable differences in Aβ_1–40 recognition by a fourth inhibitor in H₂O versus DMSO_d6.

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Article information

DOI: https://doi.org/10.1039/C5RA12100K
Article type: Communication
Submitted: 23 Jun 2015
Accepted: 10 Aug 2015
First published: 10 Aug 2015

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RSC Adv., 2015,5, 69761-69764

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DMSO affects Aβ_1–40's conformation and interactions with aggregation inhibitors as revealed by NMR

D. V. Laurents, D. Pantoja-Uceda, L. C. López, J. A. Carrodeguas, M. Mompeán, M. Á. Jiménez and J. Sancho, RSC Adv., 2015, 5, 69761 DOI: 10.1039/C5RA12100K

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