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Issue 113, 2016, Issue in Progress
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A reversible rhodamine 6G-based fluorescence turn-on probe for Fe3+ in water and its application in living cell imaging

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Abstract

A reversible fluorescent probe L based on rhodamine 6G is synthesized for the optical detection of Fe3+ in water. This probe displays favorable selectivity for Fe3+ with a 1 : 1 stoichiometry. It displays a fluorescence turn-on response in the process of rhodamine ring opening with Fe3+ and a fluorescence turn-off response with the addition of Na4P2O7 to the L-Fe3+ complex. The association constant between the probe and Fe3+ was detected to be 3.5 × 104 M−1, and the corresponding detection limit was calculated to be 1.9 × 10−8 M according to fluorescence titration analysis. Furthermore, bioimaging investigations indicated that this probe was cell permeable and suitable for monitoring intracellular Fe3+ in living cells by confocal microscopy.

Graphical abstract: A reversible rhodamine 6G-based fluorescence turn-on probe for Fe3+ in water and its application in living cell imaging

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Publication details

The article was received on 24 May 2015, accepted on 22 Jun 2015 and first published on 23 Jun 2015


Article type: Paper
DOI: 10.1039/C5RA09758D
Citation: RSC Adv., 2016,6, 111754-111759
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    A reversible rhodamine 6G-based fluorescence turn-on probe for Fe3+ in water and its application in living cell imaging

    Y. Liu, R. Shen, J. Ru, X. Yao, Y. Yang, H. Liu, X. Tang, D. Bai, G. Zhang and W. Liu, RSC Adv., 2016, 6, 111754
    DOI: 10.1039/C5RA09758D

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