One-pot synthesis of chitosan–dehydropregnenolone acetate ketimine nanoparticles and their antifungal bioevaluation

Abstract

This paper presents a new method for fabricating biodegradable bio-polymeric nanoparticles via a convenient one-pot strategy at room temperature under stirring conditions for application to communicable diseases. The simultaneous synthesis and assembly of chitosan–16-dehydropregnenolone acetate (CHDPA) nanoparticles were characterized using Fourier transform infrared (FT-IR) spectroscopy, and ¹H NMR and UV vis analyses and the morphology along-with the particle size were identified using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In this study, the antifungal properties of the CHDPA nanoparticles were tested by measuring their antifungal activity against the fungus Colletotrichum gloeosporioides. The drug loading capacity (LC), encapsulation efficiency (EE) and drug release were investigated using UV spectrophotometry.