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Issue 4, 2016
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DNA-directed spatial assembly of photosynthetic light-harvesting proteins

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Abstract

This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.

Graphical abstract: DNA-directed spatial assembly of photosynthetic light-harvesting proteins

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Publication details

The article was received on 16 Nov 2015, accepted on 09 Dec 2015 and first published on 09 Dec 2015


Article type: Paper
DOI: 10.1039/C5OB02351C
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2016,14, 1359-1362
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    DNA-directed spatial assembly of photosynthetic light-harvesting proteins

    S. L. Henry, J. M. Withers, I. Singh, J. M. Cooper, A. W. Clark, G. A. Burley and R. J. Cogdell, Org. Biomol. Chem., 2016, 14, 1359
    DOI: 10.1039/C5OB02351C

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