Issue 47, 2015
From the journal:

Organic & Biomolecular Chemistry

Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase†

Hirokazu Kage,a   Elena Riva,b   James S. Parascandolo,b   Martin F. Kreutzer,a   Manuela Tosin*b  and  Markus Nett*a  

* Corresponding authors

a Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Adolf-Reichwein-Str. 23, D-07745 Jena, Germany
E-mail: markus.nett@hki-jena.de
Fax: +49 3641 5320811
Tel: +49 3641 5321297

b Department of Chemistry, University of Warwick, Library Road, Coventry CV4 7AC, UK
E-mail: m.tosin@warwick.ac.uk
Tel: +44 (0)2476572878

Abstract

Synthetic chain terminators were used to capture the biosynthetic intermediates from a partially reducing iterative type I polyketide synthase, which is integrated into a multimodular biosynthesis enzyme. The off-loaded metabolites clarified the timing of ketoreduction and aromatization in the assembly of the antibiotic micacocidin.

Graphical abstract: Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase

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Article information

DOI
https://doi.org/10.1039/C5OB02009C
Article type
Communication
Submitted
26 Sep 2015
Accepted
13 Oct 2015
First published
28 Oct 2015
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2015,13, 11414-11417

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Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase

H. Kage, E. Riva, J. S. Parascandolo, M. F. Kreutzer, M. Tosin and M. Nett, Org. Biomol. Chem., 2015, 13, 11414 DOI: 10.1039/C5OB02009C

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