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Issue 36, 2015
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One probe, two-channel imaging of nuclear and cytosolic compartments with orange and red emissive dyes

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Abstract

Several new DNA-targeting probes that exhibit binding-induced ‘turn on’ fluorescence are presented. Two of the dyes, orange emissive 1, (E)-4-(4(-4-methylpiperazin-1-yl)phenyl)6-(4-(4-methylpi-perazin-1-yl)styryl)pyrimidin-2-ol), and red emissive 2, (E)-4-(4(-4-methyl-piperazin-1-yl)-phenyl)6-(4-(4-methylpiperazin-1-yl)styryl)-1,3-propanedionato-κOO′]difluoroborane), are brightly fluorescent when bound to DNA, but are virtually non-fluorescent in aqueous solutions. Confocal fluorescence microscopy of live BT474, MCF7 and HEK293 cells demonstrates that both probes are cell permeable and rapidly accumulated intracellularly into cell nuclei and the cytosol. Taking advantage of their environmental sensitivity, these two pools of fluorophores are readily resolved into separate channels, and thus, a single dye allows two-color imaging of the nuclear and cytosolic compartments.

Graphical abstract: One probe, two-channel imaging of nuclear and cytosolic compartments with orange and red emissive dyes

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Publication details

The article was received on 13 Jul 2015, accepted on 04 Aug 2015 and first published on 04 Aug 2015


Article type: Paper
DOI: 10.1039/C5OB01428J
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2015,13, 9477-9484
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    One probe, two-channel imaging of nuclear and cytosolic compartments with orange and red emissive dyes

    D. R. G. Pitter, A. S. Brown, J. D. Baker and J. N. Wilson, Org. Biomol. Chem., 2015, 13, 9477
    DOI: 10.1039/C5OB01428J

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