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Issue 7, 2015
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Functionalization of quantum rods with oligonucleotides for programmable assembly with DNA origami

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Abstract

The DNA-mediated self-assembly of CdSe/CdS quantum rods (QRs) onto DNA origami is described. Two QR types with unique optical emission and high polarization were synthesized, and then functionalized with oligonucleotides (ssDNA) using a novel protection-deprotection approach, which harnessed ssDNA's tailorable rigidity and denaturation temperature to increase DNA coverage by reducing non-specific coordination and wrapping. The QR assembly was programmable, and occurred at two different assembly zones that had capture strands in parallel alignment. QRs with different optical properties were assembled, opening up future studies on orientation dependent QR FRET. The QR-origami conjugates could be purified via gel electrophoresis and sucrose gradient ultracentrifugation. Assembly yields, QR stoichiometry and orientation, as well as energy transfer implications were studied in light of QR distances, origami flexibility, and conditions.

Graphical abstract: Functionalization of quantum rods with oligonucleotides for programmable assembly with DNA origami

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Publication details

The article was received on 29 Dec 2014, accepted on 12 Jan 2015 and first published on 13 Jan 2015


Article type: Communication
DOI: 10.1039/C4NR07662A
Citation: Nanoscale, 2015,7, 2883-2888
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    Functionalization of quantum rods with oligonucleotides for programmable assembly with DNA origami

    T. L. Doane, R. Alam and M. M. Maye, Nanoscale, 2015, 7, 2883
    DOI: 10.1039/C4NR07662A

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