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Issue 2, 2015
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Genetically-encoded FRET-based sensors for monitoring Zn2+ in living cells

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Abstract

Genetically-encoded fluorescent sensor proteins are attractive tools for studying intracellular Zn2+ homeostasis and signaling. Here we provide an overview of recently developed sensors based on Förster Resonance Energy Transfer (FRET). The pros and cons of the various sensors are discussed with respect to Zn2+ affinity, dynamic range, intracellular targeting and multicolor imaging. Recent applications of these sensors are described, as well as some of the challenges that remain to be addressed in future research.

Graphical abstract: Genetically-encoded FRET-based sensors for monitoring Zn2+ in living cells

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Publication details

The article was received on 04 Jul 2014, accepted on 19 Aug 2014 and first published on 19 Aug 2014


Article type: Tutorial Review
DOI: 10.1039/C4MT00179F
Author version available: Download Author version (PDF)
Citation: Metallomics, 2015,7, 258-266
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    Genetically-encoded FRET-based sensors for monitoring Zn2+ in living cells

    A. M. Hessels and M. Merkx, Metallomics, 2015, 7, 258
    DOI: 10.1039/C4MT00179F

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