Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 3, 2015
Previous Article Next Article

Proteomic analysis of the copper resistance of Streptococcus pneumoniae

Author affiliations

Abstract

Streptococcus pneumoniae is a Gram-positive bacterial pathogen causing a variety of diseases, including otitis media, bacteraemia and meningitis. Although copper is an essential trace metal for bacterial growth, high intracellular levels of free-copper are toxic. Copper resistance has emerged as an important virulence determinant of microbial pathogens. In this study, we determined the minimum inhibition concentration of copper for the growth inhibition of S. pneumoniae. Two-dimensional-electrophoresis coupled with mass spectrometry was applied to identify proteins involved in copper resistance of S. pneumoniae. In total, forty-four proteins with more than 1.5-fold alteration in expression (p < 0.05) were identified. Quantitative reverse transcription PCR was used to confirm the proteomic results. Bioinformatics analysis showed that the differentially expressed proteins were mainly involved in the cell wall biosynthesis, protein biosynthesis, purine biosynthesis, pyrimidine biosynthesis, primary metabolic process, and the nitrogen compound metabolic process. Many up-regulated proteins in response to the copper treatment directly or indirectly participated in the cell wall biosynthesis, indicating that the cell wall is a critical determinant in copper resistance of S. pneumoniae.

Graphical abstract: Proteomic analysis of the copper resistance of Streptococcus pneumoniae

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 20 Oct 2014, accepted on 13 Jan 2015 and first published on 13 Jan 2015


Article type: Paper
DOI: 10.1039/C4MT00276H
Citation: Metallomics, 2015,7, 448-454
  •   Request permissions

    Proteomic analysis of the copper resistance of Streptococcus pneumoniae

    Z. Guo, J. Han, X. Yang, K. Cao, K. He, G. Du, G. Zeng, L. Zhang, G. Yu, Z. Sun, Q. He and X. Sun, Metallomics, 2015, 7, 448
    DOI: 10.1039/C4MT00276H

Search articles by author