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Issue 14, 2015
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Self-seeding microwell chip for the isolation and characterization of single cells

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Abstract

A self-seeding microwell chip is introduced for the isolation and interrogation of single cells. A cell suspension is transferred to a microwell chip containing 6400 microwells, each microwell with a single 5 μm pore in the bottom. The fluid enters the microwell and drags a cell onto the pore. After a cell has landed onto the pore, it will stop the fluid flow through this microwell. The remaining fluid and cells will be diverted to the next available microwell. This results in a fast and efficient distribution of single cells in individual microwells. After identification by fluorescence microscopy, the cells of interest are isolated from the microwell by punching the bottom together with the cell. The overall single cell recovery of seeding followed by isolation of the single cell, is >70% with a specificity of 100% as confirmed by the genetic make-up of the isolated cells.

Graphical abstract: Self-seeding microwell chip for the isolation and characterization of single cells

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Publication details

The article was received on 13 Mar 2015, accepted on 06 Jun 2015 and first published on 08 Jun 2015


Article type: Paper
DOI: 10.1039/C5LC00304K
Citation: Lab Chip, 2015,15, 3039-3046
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    Self-seeding microwell chip for the isolation and characterization of single cells

    J. F. Swennenhuis, A. G. J. Tibbe, M. Stevens, M. R. Katika, J. van Dalum, H. Duy Tong, C. J. M. van Rijn and L. W. M. M. Terstappen, Lab Chip, 2015, 15, 3039
    DOI: 10.1039/C5LC00304K

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