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Issue 10, 2015
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Automated co-culture system for spatiotemporal analysis of cell-to-cell communication

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Abstract

We present a microfluidic co-culture system that generates localized and precisely formulated immune signals among a population of cells, enabling spatiotemporal analysis of paracrine signal transmission between different cell types. The automated system allows us to create temporally modulated chemical inputs that can be delivered to single signal-transmitting and receiving cells in a highly controlled way. Using this system we stimulated a single macrophage with brief pulses of bacterial LPS and observed the macrophage transmitted TNF signal propagating in a population of fibroblasts via NF-κB activation. The signal receiving fibroblasts transformed the TNF signal into a spatiotemporally distributed NF-κB output, recapitulating the initiation of immune response to bacterial infection.

Graphical abstract: Automated co-culture system for spatiotemporal analysis of cell-to-cell communication

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Publication details

The article was received on 13 Feb 2015, accepted on 07 Apr 2015 and first published on 08 Apr 2015


Article type: Paper
DOI: 10.1039/C5LC00182J
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 2192-2200
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    Automated co-culture system for spatiotemporal analysis of cell-to-cell communication

    T. Frank and S. Tay, Lab Chip, 2015, 15, 2192
    DOI: 10.1039/C5LC00182J

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