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Issue 8, 2015
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Modular microfluidics for point-of-care protein purifications

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Abstract

Biochemical separations are the heart of diagnostic assays and purification methods for biologics. On-chip miniaturization and modularization of separation procedures will enable the development of customized, portable devices for personalized health-care diagnostics and point-of-use production of treatments. In this report, we describe the design and fabrication of miniature ion exchange, size exclusion and affinity chromatography modules for on-chip clean-up of recombinantly-produced proteins. Our results demonstrate that these common separations techniques can be implemented in microfluidic modules with performance comparable to conventional approaches. We introduce embedded 3-D microfluidic interconnects for integrating micro-scale separation modules that can be arranged and reconfigured to suit a variety of fluidic operations or biochemical processes. We demonstrate the utility of the modular approach with a platform for the enrichment of enhanced green fluorescent protein (eGFP) from Escherichia coli lysate through integrated affinity and size-exclusion chromatography modules.

Graphical abstract: Modular microfluidics for point-of-care protein purifications

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Publication details

The article was received on 26 Jan 2015, accepted on 26 Feb 2015 and first published on 26 Feb 2015


Article type: Paper
DOI: 10.1039/C5LC00094G
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 1799-1811
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    Modular microfluidics for point-of-care protein purifications

    L. J. Millet, J. D. Lucheon, R. F. Standaert, S. T. Retterer and M. J. Doktycz, Lab Chip, 2015, 15, 1799
    DOI: 10.1039/C5LC00094G

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