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Issue 2, 2015
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A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

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Abstract

We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.

Graphical abstract: A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

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Publication details

The article was received on 22 Oct 2014, accepted on 31 Oct 2014 and first published on 31 Oct 2014


Article type: Paper
DOI: 10.1039/C4LC01259C
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 429-439
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    A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

    R. Sinha, S. Le Gac, N. Verdonschot, A. van den Berg, B. Koopman and J. Rouwkema, Lab Chip, 2015, 15, 429
    DOI: 10.1039/C4LC01259C

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