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Issue 2, 2015
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A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings

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Abstract

This paper demonstrates a microfluidic system that automates i) formation of a lipid bilayer at the interface between a pair of nanoliter-sized aqueous droplets in oil, ii) exchange of one droplet of the pair to form a new bilayer, and iii) current measurements on single proteins. A new microfluidic architecture is introduced – a set of traps designed to localize the droplets with respect to each other and with respect to the recording electrodes. The system allows for automated execution of experimental protocols by active control of the flow on chip with the use of simple external valves. Formation of stable artificial lipid bilayers, incorporation of α-hemolysin into the bilayers and electrical measurements of ionic transport through the protein pore are demonstrated.

Graphical abstract: A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings

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Publication details

The article was received on 24 Aug 2014, accepted on 05 Nov 2014, published on 06 Nov 2014 and first published online on 06 Nov 2014


Article type: Paper
DOI: 10.1039/C4LC00985A
Citation: Lab Chip, 2015,15, 541-548
  • Open access: Creative Commons BY license
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    A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings

    M. A. Czekalska, T. S. Kaminski, S. Jakiela, K. Tanuj Sapra, H. Bayley and P. Garstecki, Lab Chip, 2015, 15, 541
    DOI: 10.1039/C4LC00985A

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