Jump to main content
Jump to site search

Issue 7, 2015
Previous Article Next Article

Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation

Author affiliations

Abstract

Tocotrienols are unsaturated forms of vitamin E previously shown to reduce adipogenesis and adipose inflammation. In this study, muscadine grape seed oil (MGSO) was identified as a novel source of tocotrienols containing significant amounts of α- and γ-tocotrienol (T3) with minor seasonal changes. The aim of this study was to assess the anti-adipogenic and anti-inflammatory potential of MGSO by using primary human adipose-derived stem cells (hASCs). Differentiating hASCs were treated with MGSO and compared with rice bran and olive oil. Accumulation of triglyceride was significantly lower in MGSO-treated hASCs than rice bran and olive oils. A tocotrienol rich fraction (TRF) from MGSO was prepared by solid phase extraction and eluted with 15% 1,4-dioxane in hexane. The MGSO-derived TRF treatment significantly reduced mRNA and protein expression that are crucial to adipogenesis (e.g., PPARγ and aP2) in hASCs. Furthermore, TRF from MGSO markedly reduced LPS-induced proinflammatory gene expression in human adipocytes and cytokine secretion to the medium (IL-6 and IL-8). Collectively, our work suggests that MGSO is a stable and reliable natural source of T3 and MGSO may constitute a new dietary strategy to attenuate obesity and its associated adipose inflammation.

Graphical abstract: Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation

Back to tab navigation

Publication details

The article was received on 13 Mar 2015, accepted on 05 Jun 2015 and first published on 05 Jun 2015


Article type: Paper
DOI: 10.1039/C5FO00261C
Author version available: Download Author version (PDF)
Citation: Food Funct., 2015,6, 2293-2302
  •   Request permissions

    Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation

    L. Zhao, Y. Yagiz, C. Xu, J. Lu, S. Chung and M. R. Marshall, Food Funct., 2015, 6, 2293
    DOI: 10.1039/C5FO00261C

Search articles by author

Spotlight

Advertisements