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Issue 46, 2015
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Targeted water soluble copper–tetrazolate complexes: interactions with biomolecules and catecholase like activities

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Abstract

Two new mononuclear water soluble copper(II) complexes, [Cu{(5-pyrazinyl)tetrazolate}2(1,10-phenanthroline)] 1 and [Cu{(5-pyrazinyl)tetrazolate}(1,10-phenanthroline)2](NO3)0.5(N3)0.52, have been synthesized using the metal mediated [2 + 3] cycloaddition reaction between copper bound azide and pyrazinecarbonitrile. The interactions of these copper tetrazolate complexes 1 and 2 with biomolecules like DNA and bovine serum albumin (BSA) are studied and the catecholase like catalytic activity of compound 2 is also explored. Structural determination reveals that both compounds 1 and 2 are octahedral in nature. Screening tests were conducted to quantify the binding ability of complexes (1 and 2) towards DNA and it was revealed that complex 2 has a stronger affinity to bind to CT-DNA. DFT studies indicated that a lower HOMO–LUMO energy gap between the DNA fragment and metal complexes might be the reason for this type of stronger interaction. DNA cleavage activity was explored by gel-electrophoresis and moderate to strong DNA cleavage properties were observed in the presence and absence of co-reagents. Inhibition of cleavage in the presence of sodium azide indicates the propagation of the activity through the production of singlet molecular oxygen. Furthermore enzyme kinetic studies reflect that complex 2 is also effective in mimicking catecholase like activities. An ESI-MS spectral study indicates the probable involvement of dimeric species [(phen)2Cu-(OH)2-Cu(phen)2]2+ in the catalytic cycle.

Graphical abstract: Targeted water soluble copper–tetrazolate complexes: interactions with biomolecules and catecholase like activities

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Publication details

The article was received on 18 Apr 2015, accepted on 20 Oct 2015 and first published on 22 Oct 2015


Article type: Paper
DOI: 10.1039/C5DT01471A
Author version available: Download Author version (PDF)
Citation: Dalton Trans., 2015,44, 20154-20167
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    Targeted water soluble copper–tetrazolate complexes: interactions with biomolecules and catecholase like activities

    M. Saha, M. Das, R. Nasani, I. Choudhuri, M. Yousufuddin, H. P. Nayek, M. M. Shaikh, B. Pathak and S. Mukhopadhyay, Dalton Trans., 2015, 44, 20154
    DOI: 10.1039/C5DT01471A

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