Issue 14, 2015

A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells

Abstract

A novel two-photon fluorescent probe (HQ) for hypochlorite (ClO) has been developed based on specific ClO-promoted oxidation of a C[double bond, length as m-dash]N bond. The probe shows linear fluorescence responses to ClO with 23.5-fold enhancement. Two-photon fluorescence detection signals of HQ to ClO were observed with high selectivity and sensitivity. The good biocompatibility guaranteed the use of HQ to detect intracellular ClO under two-photon excitation. Moreover, HQ has been successfully applied to monitor the endogenously produced hypochlorite in living cells (BV-2) stimulated by lipopolysaccharide (LPS). HQ provides a new ClO detection agent for the discovery of pharmacological microglia modulators by high-throughput screening.

Graphical abstract: A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells

Supplementary files

Article information

Article type
Paper
Submitted
03 Jan 2015
Accepted
01 Mar 2015
First published
02 Mar 2015

Dalton Trans., 2015,44, 6613-6619

A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells

X. Wang, X. Wang, Y. Feng, M. Zhu, H. Yin, Q. Guo and X. Meng, Dalton Trans., 2015, 44, 6613 DOI: 10.1039/C5DT00012B

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