Issue 97, 2015
From the journal:

Chemical Communications

Surface vs. solution hybridization: effects of salt, temperature, and probe type†

Wanqiong Qiao,‡a   Hao-Chun Chiang,a   Hui Xie‡a  and  Rastislav Levicky*a  

* Corresponding authors

a Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA
E-mail: rl1306@nyu.edu
Fax: +1-718-260-3125
Tel: +1-718-260-3682

Abstract

Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.

Graphical abstract: Surface vs. solution hybridization: effects of salt, temperature, and probe type

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Article information

DOI
https://doi.org/10.1039/C5CC06674C
Article type
Communication
Submitted
09 Aug 2015
Accepted
01 Oct 2015
First published
01 Oct 2015

Chem. Commun., 2015,51, 17245-17248

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Surface vs. solution hybridization: effects of salt, temperature, and probe type

W. Qiao, H. Chiang, H. Xie and R. Levicky, Chem. Commun., 2015, 51, 17245 DOI: 10.1039/C5CC06674C

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