Issue 63, 2015

Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

Abstract

A modified sortase A recognition motif containing a masked Ni2+-binding peptide was employed to boost the efficiency of sortase-catalyzed ligation reactions. Deactivation of the Ni2+-binding peptide using a Ni2+ additive improved reaction performance at low to equimolar ratios of the glycine amine nucleophile and sortase substrate. The success of this approach was demonstrated with both peptide and protein substrates.

Graphical abstract: Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

Supplementary files

Article information

Article type
Communication
Submitted
05 Jun 2015
Accepted
02 Jul 2015
First published
02 Jul 2015

Chem. Commun., 2015,51, 12548-12551

Author version available

Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

R. David Row, T. J. Roark, M. C. Philip, L. L. Perkins and J. M. Antos, Chem. Commun., 2015, 51, 12548 DOI: 10.1039/C5CC04657B

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