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Issue 52, 2015
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Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification

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Abstract

This assay, termed branched cascade enzymatic amplification (BCEA), can be a novel and straightforward method for sensitive and specific microRNA detection in crude cellular extracts of cancer cells at physiological temperature, by coupling two ordinary polymerases, Klenow fragment exo and terminal deoxynucleotidyl transferase.

Graphical abstract: Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification

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Publication details

The article was received on 07 Apr 2015, accepted on 18 May 2015 and first published on 18 May 2015


Article type: Communication
DOI: 10.1039/C5CC02864G
Citation: Chem. Commun., 2015,51, 10543-10546
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    Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification

    B. Chi, R. Liang, L. Zhang and J. Qiu, Chem. Commun., 2015, 51, 10543
    DOI: 10.1039/C5CC02864G

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