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Issue 43, 2015
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Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

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Abstract

The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site (‘sandwich’) assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.

Graphical abstract: Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

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Publication details

The article was received on 19 Mar 2015, accepted on 24 Apr 2015 and first published on 27 Apr 2015


Article type: Communication
DOI: 10.1039/C5CC02314A
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 9050-9053
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    Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

    O. Chumphukam, T. T. Le, S. Piletsky and A. E. G. Cass, Chem. Commun., 2015, 51, 9050
    DOI: 10.1039/C5CC02314A

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