Issue 41, 2015
From the journal:

Chemical Communications

A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels†

Nina Lülsdorf,‡a   Christian Pitzler,‡b   Michael Biggel,b   Ronny Martinez,c   Ljubica Vojcicb  and  Ulrich Schwaneberg*ab  

* Corresponding authors

a DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056 Aachen, Germany

b Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany
E-mail: u.schwaneberg@biotec.rwth-aachen.de
Fax: +49 241 80 22387
Tel: +49 241 80 24170

c EW-Nutrition GmbH, Nattermannallee 1, 50829 Köln, Germany

Abstract

A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3–7-fold) for three hydrolases (esterase, lipase, cellulase). The screening principle is based on coupled enzymatic reaction using glucose derivatives which yield upon hydrolysis a fluorescent-hydrogel-layer on the surface of E. coli cells.

Graphical abstract: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels

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Article information

DOI
https://doi.org/10.1039/C5CC01791B
Article type
Communication
Submitted
02 Mar 2015
Accepted
16 Apr 2015
First published
16 Apr 2015

Chem. Commun., 2015,51, 8679-8682

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A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels

N. Lülsdorf, C. Pitzler, M. Biggel, R. Martinez, L. Vojcic and U. Schwaneberg, Chem. Commun., 2015, 51, 8679 DOI: 10.1039/C5CC01791B

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