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Issue 23, 2015
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Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

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Abstract

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene–cyanoacetamide moiety, were tethered to 2′-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.

Graphical abstract: Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

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Publication details

The article was received on 19 Jan 2015, accepted on 10 Feb 2015 and first published on 11 Feb 2015


Article type: Communication
DOI: 10.1039/C5CC00530B
Citation: Chem. Commun., 2015,51, 4880-4882
  • Open access: Creative Commons BY license
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    Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

    D. Dziuba, R. Pohl and M. Hocek, Chem. Commun., 2015, 51, 4880
    DOI: 10.1039/C5CC00530B

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