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Issue 15, 2015
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Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

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Abstract

We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

Graphical abstract: Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

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Publication details

The article was received on 06 Dec 2014, accepted on 07 Jan 2015 and first published on 23 Jan 2015


Article type: Communication
DOI: 10.1039/C4CC09765C
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 3266-3269
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    Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

    S. V. Nair, M. A. Witek, J. M. Jackson, M. A. M. Lindell, S. A. Hunsucker, T. Sapp, C. E. Perry, M. L. Hupert, V. Bae-Jump, P. A. Gehrig, W. Z. Wysham, P. M. Armistead, P. Voorhees and S. A. Soper, Chem. Commun., 2015, 51, 3266
    DOI: 10.1039/C4CC09765C

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