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Issue 22, 2015
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Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers

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Abstract

Mass spectrometry-based methods for prostate cancer biomarker discovery are hampered by their low-throughput capabilities because of extensive sample preparation. We present the parafilm-assisted microdissection technique coupled with label-free quantification and bioinformatics analysis as a means to evaluate directly protein expression changes on benign and tumor regions.

Graphical abstract: Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers

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Publication details

The article was received on 21 Oct 2014, accepted on 02 Dec 2014 and first published on 03 Dec 2014


Article type: Communication
DOI: 10.1039/C4CC08331H
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 4564-4567
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    Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers

    J. Quanico, J. Franck, J. P. Gimeno, R. Sabbagh, M. Salzet, R. Day and I. Fournier, Chem. Commun., 2015, 51, 4564
    DOI: 10.1039/C4CC08331H

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