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Issue 25, 2015
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Collagen labelling with an azide-proline chemical reporter in live cells

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Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

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Publication details

The article was received on 08 Oct 2014, accepted on 17 Nov 2014 and first published on 18 Nov 2014


Article type: Communication
DOI: 10.1039/C4CC07974D
Citation: Chem. Commun., 2015,51, 5250-5252
  • Open access: Creative Commons BY license
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    Collagen labelling with an azide-proline chemical reporter in live cells

    B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250
    DOI: 10.1039/C4CC07974D

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