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Issue 2, 2015
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Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

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Abstract

Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.

Graphical abstract: Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

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Publication details

The article was received on 01 Oct 2014, accepted on 30 Oct 2014 and first published on 04 Nov 2014


Article type: Communication
DOI: 10.1039/C4CC07778D
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2015,51, 409-412
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    Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

    F. J. Piscotta, J. M. Tharp, W. R. Liu and A. J. Link, Chem. Commun., 2015, 51, 409
    DOI: 10.1039/C4CC07778D

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