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Issue 10, 2015
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Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

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Abstract

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N′-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a “naïve” state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.

Graphical abstract: Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

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Publication details

The article was received on 13 Apr 2015, accepted on 11 Jul 2015 and first published on 22 Jul 2015


Article type: Communication
DOI: 10.1039/C5BM00121H
Author version available: Download Author version (PDF)
Citation: Biomater. Sci., 2015,3, 1371-1375
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    Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

    C. Mangani, A. Lilienkampf, M. Roy, P. A. de Sousa and M. Bradley, Biomater. Sci., 2015, 3, 1371
    DOI: 10.1039/C5BM00121H

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